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目的:探讨正常子宫内膜、子宫内膜非典型增生和子宫内膜腺癌组织环氧化酶-2(COX-2)和增殖细胞核抗原(PCNA)的表达及与临床、病理的关系。方法:应用SP法检测正常子宫内膜、子宫内膜非典型增生和子宫内膜腺癌组织中COX-2和PCNA的表达。结果:正常子宫内膜中COX-2和PCNA的阳性表达率或高增殖率分别为15.0%和10.0%;非典型增生内膜中COX-2和PCNA的阳性表达率或高增殖率分别为44.4%和11.1%;子宫内膜癌组织中COX-2和PCNA阳性表达率或高增殖率为66.7%和54.4%。COX-2与肿瘤组织的手术-病理分期、分化程度及肌层浸润相关。PCNA在子宫内膜腺癌中表达与肿瘤组织的手术-病理分期、分化程度及淋巴结转移相关。COX-2表达阳性子宫内膜腺癌中PCNA高增殖率为76.3%,COX-2表达阴性子宫内膜腺癌中PCNA高增殖率为10.5%,两者呈正相关。结论:①COX-2和PCNA异常表达与子宫内膜癌的发生、发展密切相关,两者可能在子宫内膜由良性到恶性的转变过程中,以及恶性肿瘤进展中起了重要作用。②COX-2的表达和PCNA的表达呈正相关,提示两者有协同的作用增强癌细胞的增殖活性。
Objective: To investigate the expression of cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) in normal endometrium, endometrial atypical hyperplasia and endometrial adenocarcinoma and their relationship with clinical and pathological changes. Methods: The expressions of COX-2 and PCNA in normal endometrium, endometrial dysplasia and endometrial adenocarcinoma were detected by SP method. Results: The positive expression rates of COX-2 and PCNA in normal endometrium were 15.0% and 10.0%, respectively. The positive expression rate of COX-2 and PCNA in atypical hyperplasia endometrium were 44.4 % And 11.1% respectively. The positive rates of COX-2 and PCNA in endometrial carcinoma tissues were 66.7% and 54.4%, respectively. COX-2 was associated with tumor-pathological stage, differentiation and myometrial invasion. The expression of PCNA in endometrial adenocarcinoma is related to the tumor-pathological stage, differentiation and lymph node metastasis. The high proliferative rate of PCNA in COX-2 positive endometrial adenocarcinoma was 76.3%, while the high proliferative rate of PCNA in COX-2 negative endometrial adenocarcinoma was 10.5%, which showed a positive correlation. Conclusion: (1) The abnormal expression of COX-2 and PCNA is closely related to the occurrence and development of endometrial carcinoma, both of which may play an important role in the process of benign to malignant transformation of endometrium and the progression of malignant tumor. ②COX-2 expression and PCNA expression was positively correlated, suggesting that both have a synergistic effect to enhance the proliferation of cancer cells.