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目的通过模拟体内代谢,对双酚A二缩水甘油醚(BADGE)的体外基本代谢情况进行研究。方法采用肝微粒体、肝S9 2种体外代谢试剂,通过模拟体内肝脏代谢,对BADGE的代谢行为及其代谢产物进行研究。通过代谢试剂浓度及代谢时间条件的优化,采用高效液相色谱-串联质谱(HPLC-MS/MS)作为检测手段对BADGE的体外代谢产物进行分析确证。结果体外代谢最佳孵化时间为60 min,最佳体外代谢试剂浓度为0.5mg/m L,在肝S9及肝微粒体2种体外代谢试剂的作用下,BADGE发生显著的代谢反应。结论本研究与传统的动物试验相比,节约了时间、精力,对食品包装材料的毒理学研究和安全性评价有重要的推动作用。
Objective To study the basic metabolism of bisphenol A diglycidyl ether (BADGE) in vitro by simulating the metabolism in vivo. Methods Metabolic agents of liver microsome and liver S9 were used to study the metabolic behavior of BADGE and its metabolites by simulating liver metabolism in vivo. Through the optimization of metabolic reagent concentration and metabolic time, the metabolites of BADGE in vitro were analyzed and confirmed by HPLC-MS / MS. Results The best in vitro metabolism incubation time was 60 min and the best in vitro metabolite concentration was 0.5 mg / mL. There was a significant metabolic reaction of BADGE under the action of two in vitro metabolic reagents of liver S9 and liver microsomes. Conclusion Compared with traditional animal experiments, this study saved time and energy and played an important role in the toxicology research and safety evaluation of food packaging materials.