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目的:研究活血行气方健脾理气方对人胃癌SGC-7901细胞株p53和Bcl-2基因表达的影响。方法:利用血清药理学方法制备药物血清,应用Western-blot分析方法观察活血行气方对人胃癌SGC-7901细胞株p53、Bcl-2基因表达的影响。结果:药物血清作用后,p53表达明显增强;Bcl-2表达明显降低。结论:活血行气方药物血清上调p53、下调Bcl-2,达到抑制人胃癌SGC-7901细胞株的作用。
Objective: To study the effect of invigorating blood circulation Qi and invigorating the spleen Qi prescription on p53 and Bcl-2 gene expression in human gastric cancer cell line SGC-7901. Methods: Serum pharmacological methods were used to prepare the serum of patients. The effects of Huoxue Xingqi Recipe on the gene expression of p53 and Bcl-2 in human gastric cancer cell line SGC-7901 were observed by Western-blot analysis. Results: The effect of serum on the expression of p53 was significantly enhanced; the expression of Bcl-2 was significantly decreased. CONCLUSION: The serum of Huoxue Xingqi recipe up-regulates p53 and down-regulates Bcl-2, which can inhibit the growth of human gastric cancer cell line SGC-7901.