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目的:通过比较乳腺癌患者与正常人群及女性肺癌患者外周血淋巴细胞10种表型的变化,探讨淋巴细胞亚群检测对乳腺癌患者的临床应用价值。方法:采用四色免疫荧光标记流式细胞仪分析技术。结果:与正常对照组比较,乳腺癌组CD3+、CD4+细胞百分数及CD4+/CD8+比值明显下降(P=0.000 0,P=0.036 1),而CD8+和CD3+CD16+CD56+细胞两组间比较差异无统计学意义(P=0.959 4,P=0.353 2),静止总T淋巴细胞比例下降(P=0.018 9)、活化B淋巴细胞和调节性T细胞比例升高(P=0.000 0,P=0.002 3);与女性肺癌组比较,乳腺癌组CD3+细胞和CD4+细胞明显下降(P=0.0021,P=0.000 2);CD8+细胞、CD3-CD16+CD56+细胞和CD4+/CD8+比值2组间比较差异无统计学意义(P=0.413 6,P=0.800 8,P=0.413 6),静止总T淋巴细胞和抑制性T细胞比例降低(P=0.010 2,P=0.021 7),活化总T淋巴细胞、活化B淋巴细胞、细胞毒性T细胞和调节性T细胞均升高(P=0.000 0,P=0.000 6,P=0.000 0,P=0.002 9)。结论:这些受检乳腺癌患者的免疫功能下降明显。但是可能仍处于代偿阶段,并未受到深度抑制。
Objective: To compare the ten phenotypes of peripheral blood lymphocytes in patients with breast cancer, normal controls and female patients with lung cancer, and to explore the clinical value of detecting lymphocyte subsets in patients with breast cancer. Methods: Four-color immunofluorescence labeled flow cytometry analysis. Results: Compared with the normal control group, the percentage of CD3 +, CD4 + cells and the ratio of CD4 + / CD8 + decreased significantly in breast cancer group (P = 0.0000, P = 0.0361), but there was no difference between the two groups in CD8 + and CD3 + CD16 + CD56 + cells Statistically significant (P = 0.959 4, P = 0.353 2), decreased proportion of resting T lymphocytes (P = 0.018 9), increased proportion of activated B lymphocytes and regulatory T cells (P = 0.000 0, P = 0.002 3). Compared with female lung cancer group, the expression of CD3 + cells and CD4 + cells in breast cancer group was significantly decreased (P = 0.0021, P = 0.0002). There was no significant difference in the ratio of CD8 + CD3 + CD16 + CD56 + cells and CD4 + / CD8 + The proportion of total T lymphocytes and suppressor T cells decreased (P = 0.010 2, P = 0.021 7), total T lymphocytes were activated, Activated B lymphocytes, cytotoxic T cells and regulatory T cells were elevated (P = 0.000 0, P = 0.0006, P = 0.000 0, P = 0.002 9). Conclusion: The immune function of these patients with breast cancer decreased significantly. However, it may still be at a compensatory stage and has not been deeply suppressed.