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目的探讨十溴联苯醚(PBDE-209)对宫颈癌HeLa细胞体外增殖的影响。方法观察HeLa细胞经不同浓度PBDE-209暴露后发生的形态学变化,并用MTT方法检测细胞存活率改变,采用免疫荧光化学方法检测细胞增殖核抗原Ki67表达变化,流式细胞术观察HeLa细胞细胞周期的分布变化。结果随着浓度升高,细胞增殖明显,细胞间隙变小。MTT方法检测发现PBDE-209促进HeLa细胞增殖,细胞增殖率的升高呈时间和浓度依赖效应,200nmol/LPBDE-209作用24、48和72h后细胞增殖率分别是阴性对照组的1.2、1.5和1.8倍(P<0.05)。Ki67表达强度随着PBDE-209浓度升高而增强。流式细胞术检测发现200nmol/LPBDE-209作用72h后,G0/G1期细胞比例下降了8%,而S期细胞比例升高了7%,与阴性对照组比较差异均有统计学意义(P<0.05)。结论低浓度PBDE-209暴露使HeLa细胞G0/G1期细胞减少,S期细胞增加,从而促进细胞增殖。
Objective To investigate the effects of decabromodiphenyl ether (PBDE-209) on the proliferation of cervical cancer HeLa cells in vitro. Methods The morphological changes of HeLa cells exposed to different concentrations of PBDE-209 were observed. The cell viability was detected by MTT assay. Ki67 expression was detected by immunofluorescence staining. The cell cycle of HeLa cells was observed by flow cytometry The distribution of changes. Results With the increase of concentration, cell proliferation was obvious and cell gap became smaller. MTT assay showed that PBDE-209 promoted the proliferation of HeLa cells in a time and concentration-dependent manner. The proliferation rates of cells treated with 200nmol / LPBDE-209 for 24, 48 and 72h were 1.2, 1.5 and 1.8 times (P <0.05). Ki67 expression intensity increased with increasing PBDE-209 concentration. Flow cytometry showed that the proportion of cells in G0 / G1 phase decreased by 8% and the proportion of cells in S phase increased by 7% after 72h treatment with 200nmol / LPBDE-209, the difference was statistically significant compared with the negative control group (P <0.05). Conclusion Exposure of PBDE-209 to low concentration can decrease the number of G0 / G1 phase cells and increase the number of S phase cells in HeLa cells, thereby promoting cell proliferation.