获取旋毛虫肌幼虫排泄分泌（ＥＳ）抗原的部分结构基因并进行了克隆、鉴定和表达。首先采用ＲＴＰＣＲ技术从旋毛虫肌幼虫总ＲＮＡ中获取目的基因，经酶切分析后与融合表达载体ｐＥＸ３１Ｃ、ｐＥＸ３１Ｂ及非融合表达载体ｐＢＶ２２０连接，并将其转入大肠杆菌进行表达。结果：重组质粒在大肠杆菌中表达出相应的相对分子质量的重组蛋白，经纯化后的重组蛋白可与旋毛虫病猪阳性血清发生反应。提示：重组抗原可作为ＥＳ抗原的候选替代抗原 The partial structural genes of the excretory-secretory (ES) antigen of Trichinella spiralis muscle larvae were obtained and cloned, identified and expressed. Firstly, RT-PCR was used to obtain the target gene from total RNA of Trichinella spiralis larvae. After digested with restriction enzyme, the fusion gene was ligated with pEX31C, pEX31B and non-fusion expression vector pBV220 and transformed into E.coli. Results: The recombinant plasmids were expressed in Escherichia coli with the corresponding molecular weight of the recombinant protein, and the purified recombinant protein reacted with the positive serum of Trichinella. Tip: Recombinant antigens can be used as a candidate antigen instead of ES antigen