中华绒鳌蟹窦腺组织个体很小,RNA含量较少,利用TRIzol一步法提取其总RNA效果较差,且解剖过程中RNA易降解。本文对TRIzol试剂盒说明书的提取过程进行了适当改进,有效提高了窦腺总RNA的产率,减少了RNA降解量,延长了RNA的储存时间。该方法对于低丰度RNA提取尤为有效。 Eriocheir sinensis sinus gland tissue is small, RNA content less, the use of TRIzol one-step extraction of total RNA less effective, and the degradation of RNA during the dissection. In this paper, the extraction process of TRIzol kit instructions was appropriately improved, which effectively improved the yield of total RNA of sinus glands, reduced the amount of RNA degradation and prolonged the storage time of RNA. This method is particularly effective for low abundance RNA extraction.