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研究频率为50Hz不同强度正弦交变电磁场(SEMFs)对大鼠峰值骨量(PBM)的影响,为电磁场临床试验提供理论依据。将30只6周龄SD实验大鼠随机分为3组(n=10),对照组、0.1mT磁场组和0.6mT磁场组。磁场组分别给予不同强度磁场干预3h/d、8周后,检测骨密度(BMD)、生物力学、血清骨钙素(OC)和血清抗酒石酸酸性磷酸酶5b(TRACP 5b)以及以stevenol蓝品红苦味酸染色(VG)进行骨形态分析。实验结果表明,0.1mT磁场组大鼠全身骨密度、股骨、椎骨骨密度和股骨最大载荷显著高于对照组(P<0.01),股骨和椎骨屈服强度及血清生化指标显著优于对照组(P<0.05),骨小梁宽度、骨小梁数目均有增加,骨小梁间隙减小。0.6mT组与对照组相比,差异无统计意义(P>0.05)。说明50Hz 0.1mT电磁场能增加SD大鼠骨密度,改善骨组织微结构,增强骨强度,从而提高大鼠的峰值骨量。
The effect of SEMFs with different intensities on the peak bone mass (PBM) of rats at 50 Hz was studied, which provided a theoretical basis for clinical trials of electromagnetic fields. Thirty 6-week-old SD rats were randomly divided into three groups (n = 10), control group, 0.1mT magnetic field group and 0.6mT magnetic field group. Magnetic field were given different intensity magnetic field intervention 3h / d, 8 weeks after the detection of bone mineral density (BMD), biomechanics, serum osteocalcin (OC) and serum anti-tartrate acid phosphatase 5b (TRACP 5b) Red picric acid staining (VG) for bone morphological analysis. The experimental results showed that the whole body BMD, the BMD of femur and vertebra and the maximum load of femur in 0.1mT magnetic field group were significantly higher than those in control group (P <0.01). The yield strength and serum biochemical indexes of femur and vertebra were significantly better than those in control group (P <0.05), trabecular width, the number of trabecular increased, trabecular clearance decreased. 0.6mT group compared with the control group, the difference was not statistically significant (P> 0.05). It shows that 50Hz 0.1mT electromagnetic field can increase the bone density of SD rats, improve the microstructure of bone tissue and enhance the bone strength, so as to increase the peak bone mass of rats.