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雄性成年大鼠36只,3只为正常对照;33只做肝大部切除。切除分11组,每组3例。分别于术后12～120小时内的不同间隔时间取材。动物经门静脉灌注去血,分离制备肝细胞悬液,荧光(EB)染色,用国产流式细胞计(FCM)测定肝细胞DNA含量,以鸡红细胞为内插标准,每例动物测5～6×10~4个肝细胞。同时制作肝组织切片和肝细胞涂片,分别计数肝细胞分裂指数(MI)和双核肝细胞数。正常大鼠肝细胞DNA含量有2 n、4 n、8 n组型,以4 n肝细胞占优势(76%)。肝大部切除后20小时,部分4 n肝细胞快速分裂,2 n肝细胞比例增加,双核肝细胞减少。术后24小时出现4 n肝细胞S期(平均为11.5%)和G_2期(8 n)高峰。8 n肝细胞为
Thirty-six male adult rats were used as normal control and 33 as partial hepatectomy. Excision of 11 groups, 3 cases in each group. Respectively within 12 to 120 hours after surgery at different intervals drawn. The animals were perfused by portal vein to remove the blood, the isolated and prepared hepatocyte suspension was stained with fluorescent (EB). The DNA content of hepatocytes was determined by domestic flow cytometry (FCM) × 10 ~ 4 hepatocytes. At the same time, liver sections and hepatocyte smears were made and counted for hepatocyte division index (MI) and binuclear hepatocyte count. The DNA content of normal rat hepatocytes was 2n, 4n, 8n and dominated by 4n hepatocytes (76%). Twenty hours after partial hepatectomy, some 4n hepatocytes rapidly divide, the proportion of 2n hepatocytes increases, and the number of binucleated hepatocytes decreases. At 4 hours after operation, 4n hepatocytes showed S phase (average 11.5%) and G 2 phase (8 n) peak. 8 n liver cells