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利用降解菌HN36降解土壤中的二氯喹啉酸及修复土壤的生态环境,为稻田植烟地区烟叶安全生产提供技术和理论依据。采用盆栽试验,将二氯喹啉酸与降解菌HN36配成一定量的溶液,均匀喷洒到过筛的干土中,将5叶期烟苗移栽到处理的塑料盆土中。当烟苗长到8叶期时分别取茎尖﹑顶叶和中部叶进行处理,在电镜下观察细胞超微结构变化,利用高效液相色谱仪检测土壤中二氯喹啉酸降解的动态。烟株细胞超微结构变化的程度有差异,表现为受害烟株>修复烟株>健康烟株;烟株的茎尖和顶叶细胞伤害最严重,中部叶片细胞伤害较轻。研究结果还显示,在含二氯喹啉酸的土壤中,加入降解菌HN36能加速二氯喹啉酸降解,改善土壤健康质量,烟株细胞超微结构及烟叶品质明显得到修复。
Degradation bacteria HN36 degraded quinclorac in soil and rehabilitated the ecological environment of the soil, providing technical and theoretical basis for tobacco leaf safety in the tobacco planting areas. In pot experiment, quinclorac and detoxifying bacteria HN36 were mixed into a certain amount of solution, sprayed evenly on the sieved dry soil, and 5-leaf stage tobacco seedlings were transplanted to the treated plastic basin soil. When the tobacco leaves grew to the 8-leaf stage, the shoot tip, the parietal lobe and the middle leaf were taken for treatment. The ultrastructural changes of the cells were observed under electron microscope. The degradation of quinclorac in the soil was detected by high performance liquid chromatography. There were differences in the ultrastructural changes of tobacco plant cells, which showed that the infected tobacco plants> the repaired tobacco plants> the healthy tobacco plants. The stem and parietal cells of the tobacco plants harmed most seriously, and the damage of the middle leaves was lighter. The results also showed that addition of degrading bacteria HN36 could accelerate the degradation of quinclorac and improve soil health quality in quinclorac-containing soil, and the ultrastructure of tobacco plants and the quality of tobacco leaf were obviously repaired.