植物有机营养试验中供试无菌苗通常由种子获得 ,因此 ,种子灭菌非常重要 .采用多种消毒剂( 70 %酒精 ,10 % H2 O2 ,2 %次氯酸钠和 0 .1% Hg Cl2 等 )对几种作物种子进行组合灭菌 .结果表明 ,灭菌效果与作物种类、种子贮存时间及不同消毒剂组合等有很大关系 .采用 70 %酒精浸 1min→无菌水冲3次→ 2 %次氯酸钠浸 5min→无菌水冲 5次→ 0 .1% Hg Cl2 浸 5min→无菌水冲 6次的组合灭菌程序 ,可取得较好的灭菌效果 ;若种子对 2 %次氯酸钠敏感 ,则可用 10 % H2 O2 代替 Sterile seedlings are usually obtained from seed in plant organic nutrition tests and therefore it is important to sterilize the seeds using various disinfectants (70% alcohol, 10% H 2 O 2, 2% sodium hypochlorite and 0.1% Hg Cl 2, etc.) The results showed that the effect of sterilization was closely related to crop species, seed storage time and different combinations of disinfectants.Using 70% alcohol for 1min → sterile water for 3 times → 2% Sodium hypochlorite immersion 5min → sterile water rinse 5 → 0 .1% Hg Cl2 dipped 5min → sterile water 6 times the combination of sterilization procedures, can achieve better sterilization effect; if the seed is sensitive to 2% sodium hypochlorite, then Available 10% H2 O2 instead