论文部分内容阅读
表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)是茶叶活性物质主要成分.EGCG可预防或治疗多种肿瘤.本文旨在探讨EGCG对人乳腺癌MDA-MB-231细胞增殖、凋亡及迁移力的作用及其机制.经EGCG处理后,通过流式细胞术及噻唑蓝(MTT)法发现,EGCG使MDAMB-231细胞周期阻滞,细胞凋亡数量上升,明显抑制乳腺癌细胞的存活率.EGCG处理后,MDAMB-231细胞的形态由正常的纤维状变为鹅卵石状.免疫荧光染色及免疫印迹结果表明,其上皮细胞标志物表达量增加,而间质标志物表达量下降.通过划痕实验发现,EGCG明显抑制了细胞迁移能力.本文揭示了EGCG通过抑制乳腺癌MDA-MB-231细胞周期进程,促进间质-上皮转化,抑制乳腺癌细胞增殖和迁移.
Epigallocatechin-3-gallate (EGCG) is the main component of tea active substances. EGCG can prevent or treat various tumors. This article aims to investigate the proliferation of human breast cancer MDA-MB-231 cells by EGCG, Apoptosis and migration mechanism and its mechanism. After treated with EGCG, flow cytometry and thiazolyl blue (MTT) method found that EGCG MDAMB-231 cell cycle arrest, increased cell apoptosis, significantly inhibited breast cancer Survival rate of cells: After treatment with EGCG, the morphology of MDAMB-231 cells changed from normal fibroids to cobblestones. Immunofluorescence staining and Western blot results showed that the expression of epithelial cell markers increased and the amount of interstitial markers expressed. Decreased. Scratch test found that EGCG significantly inhibited cell migration. This study revealed that EGCG inhibits cell cycle progression of breast cancer MDA-MB-231, promotes mesenchymal-epithelial transformation, and inhibits breast cancer cell proliferation and migration.