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目的:通过检测HSV-2在子宫颈癌细胞和非癌变细胞的感染情况及HSV-2感染的子宫颈癌组中增殖细胞核抗原(PCNA)和Fas、FasL蛋白的表达,探讨HSV-2对子宫颈细胞增殖与凋亡的影响,为研究子宫颈癌的病毒致癌机理提供参考。方法:用免疫组织化学染色方法(SP法)检测80例子宫颈癌、30例慢性宫颈炎的子宫颈上皮细胞HSV-2的表达及HSV-2阳性子宫颈癌组中PCNA、Fas、FasL的表达,采用医学统计学方法分析各种产物表达的关系。结果:子宫颈癌组HSV-2的阳性表达率为63.75%(51/80),明显高于慢性宫颈炎组的40%(12/30)(P<0.05)。HSV-2阳性子宫颈癌组PCNA的阳性表达率为94.11%,明显高于HSV-2阴性组的79.31%,二者正相关(r1=0.225,P1=0.045<0.05);HSV-2阳性子宫颈癌组Fas蛋白的阳性表达率为41.18%,明显低于HSV-2阴性组的75.86%,二者负相关(r1=?0.334,P1=0.002<0.05);HSV-2阳性子宫颈癌组FasL蛋白的阳性表达率为84.31%,明显高于HSV-2阴性组的58.62%,二者正相关(r1=0.285,P1=0.01<0.05)。结论:子宫颈癌的发生与HSV-2感染相关。HSV-2感染可能通过使子宫颈上皮细胞PCNA表达上调,并抑制子宫颈癌变细胞Fas的表达,促进细胞过度增殖并使凋亡减弱而致癌。HSV-2可能会使子宫颈癌变细胞FasL表达上调,干扰机体免疫细胞活性及免疫监控,使子宫颈癌细胞逃逸来自机体的免疫杀伤。
OBJECTIVE: To detect the HSV-2 infection in cervical cancer cells and non-cancerous cells and the expression of proliferating cell nuclear antigen (PCNA) and Fas, FasL in HSV-2-infected cervical cancer group Cervical cell proliferation and apoptosis, provide a reference for the study of the carcinogenic mechanism of cervical cancer virus. Methods: The expression of HSV-2 in 80 cases of cervical cancer and 30 cases of chronic cervicitis and the expression of PCNA, Fas and FasL in HSV-2 positive cervical cancer were detected by immunohistochemical staining (SP method) , The use of medical statistics method to analyze the relationship between the expression of various products. Results: The positive rate of HSV-2 in cervical cancer was 63.75% (51/80), significantly higher than that in chronic cervicitis (40%, 12/30) (P <0.05). The positive rate of PCNA in HSV-2 positive cervical cancer group was 94.11%, which was significantly higher than 79.31% in HSV-2 negative group (r1 = 0.225, P1 = 0.045 <0.05) The positive expression rate of Fas protein in cervical cancer group was 41.18%, which was significantly lower than that in HSV-2 negative group (r = 0.334, P1 = 0.002 <0.05); HSV-2 positive cervical cancer group The positive expression rate of FasL protein was 84.31%, which was significantly higher than 58.62% in HSV-2 negative group (r1 = 0.285, P1 = 0.01 <0.05). Conclusion: The incidence of cervical cancer is associated with HSV-2 infection. HSV-2 infection may cause cancer by up-regulating the expression of PCNA in cervical epithelial cells, inhibiting the expression of Fas in uterine cervix cancer cells, promoting cell proliferation and reducing apoptosis. HSV-2 may up-regulate the expression of FasL in cervical cancer cells, interfere with the activity of immune cells and monitor the immune system, so that cervical cancer cells escape immune-killing from the body.