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目的:探讨激活处理对人未受精卵子和早期胚胎三磷酸腺苷(ATP)含量影响。方法:收集辅助生殖治疗周期中体外受精(IVF)以及单精子卵母细胞浆内注射(ICSI)时的未受精卵子(包括GV期、MⅠ期、MⅡ期卵母细胞),以及早期胚胎(2-cell、4-cell胚胎),采用生物化学发光法测定在未处理和进行激活处理后的ATP含量。结果:1未处理的GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎的ATP含量分别为1.14±0.15、1.41±0.14、1.45±0.12、1.61±0.25、2.05±0.23 pmol,激活处理后GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎的ATP含量分别为1.60±0.17、1.86±0.25、2.06±0.38、2.26±0.53、2.55±0.46 pmol;2激活处理后GV期、MⅠ期卵子的ATP含量显著性高于未处理组(P<0.05)。结论:人GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎中ATP含量逐渐增加;经激活处理后,仅GV期、MⅠ期卵子的ATP含量有显著性变化。
Objective: To investigate the effects of activation treatment on ATP content in human unfertilized and early embryos. Methods: Unfertilized eggs (including GV, MⅠ and MⅡ oocytes) during in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) of assisted spermatogenesis were collected, and early embryos (2 -cell, 4-cell embryos), the biochemical luminescence was used to determine the ATP content after untreated and activated treatment. Results: The untreated GV, MⅠ, MⅡ and 2-cell and 4-cell embryos showed ATP contents of 1.14 ± 0.15, 1.41 ± 0.14, 1.45 ± 0.12, 1.61 ± 0.25, 2.05 ± 0.23 pmol , ATP content of GV, MⅠ, MⅡ and 2-cell and 4-cell embryos were 1.60 ± 0.17,1.86 ± 0.25,2.06 ± 0.38,2.26 ± 0.53,2.55 ± 0.46 pmol after activation After GV activation, the ATP content of M Ⅰ oocytes was significantly higher than that of the untreated group (P <0.05). CONCLUSION: The ATP content of human GV, MⅠ, MⅡ and 2-cell, 4-cell embryos increases gradually. After being activated, the content of ATP in M Ⅰ egg has significant changes only in GV phase.