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目的检测γ-干扰素在艾滋病病毒(HIV)合并结核分枝杆菌(TB)感染者中的表达情况。方法选择2014—2015年5月31日于浙江省青春医院感染科住院的25例HIV合并TB感染者(HIV/TB组),以30例单纯HIV感染者(HIV组)及30例单纯结核分枝杆菌感染者(TB组)作为实验对照,采用γ-干扰素释放试验(IGRA)检测γ-干扰素水平,分析抗结核治疗和CD4+T淋巴细胞计数对γ-干扰素水平的影响。结果 HIV/TB组的IGRA阳性率为84.00%,高于HIV组的6.67%(P<0.05),与TB组的86.67%比较,差异无统计学意义(P>0.05);HIV/TB组抗结核治疗前后的IGRA阳性率比较,差异无统计学意义(P>0.05),抗结核治疗后γ-干扰素平均水平(80.12±72.01)pg/m L低于治疗前的(158.88±104.06)pg/m L(P<0.05);HIV/TB组在抗结核治疗前按CD4+T淋巴细胞数分为﹤200/μL组与≥200/μL组,两组IGRA阳性率及γ-干扰素水平比较差异均无统计学意义(P>0.05)。结论γ-干扰素在HIV合并TB感染者中有较高表达,并且γ-干扰素水平在抗结核治疗过程中呈现下降趋势,但其与CD4+T淋巴细胞计数未见相关性。
Objective To detect the expression of γ-interferon in HIV-infected patients with Mycobacterium tuberculosis (TB) infection. Methods Twenty-five HIV-TB patients (HIV / TB group) admitted to the Infectious Diseases Section of Zhejiang Youth Hospital from May 2014 to May 31,2015 were enrolled in this study. Thirty cases of HIV-infected individuals (HIV group) and 30 patients with simple tuberculosis Interferon-γ (IFN-γ) levels were determined by interferon-γ release assay (IGRA) and the effects of anti-TB therapy and CD4 + T lymphocyte count on IFN-γ level were analyzed. Results The positive rate of IGRA in HIV / TB group was 84.00%, which was significantly higher than that in HIV group (6.67%, P <0.05), but not significantly different from that in TB group (86.67%, P> 0.05) There was no significant difference in the positive rate of IGRA before and after TB treatment (P> 0.05). The average level of IFN-γ (80.12 ± 72.01) pg / m L after antituberculosis treatment was lower than that before treatment (158.88 ± 104.06) pg / m L (P <0.05). The HIV / TB group was divided into <200 / μL group and ≥200 / μL group according to the number of CD4 + T lymphocytes before antituberculous treatment. The positive rate of IGRA and the level of interferon- There was no significant difference between the two groups (P> 0.05). Conclusions γ-interferon is highly expressed in HIV-infected TB patients, and the level of IFN-γ shows a decreasing trend in the course of anti-TB treatment, but it has no correlation with CD4 + T lymphocyte count.