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目的:探索雪腐镰刀菌烯酸(NIV)对培养软骨细胞代谢的影响及硒的保护作用。方法:应用生物化学方法检测NIV对培养的单层软骨细胞代谢的影响,软骨细胞单层培养、应用琼脂糖电泳和碱性单细胞微量凝胶电泳检测培养的单层软骨细胞DNA的损伤。结果:在实验设定的NIV浓度范围内(0.0005～0.0200 mg/L),随NIV浓度增加,软骨细胞基质蛋白多糖代谢明显降低、蛋白质合成减少、DNA含量减少、DNA受损细胞增多、DNA损伤加重,同浓度NIV加硒组损伤情况较未加硒组轻。在设定3个NIV浓度下,NIV并不影响软骨细胞的脂质过氧化,但加硒可使脂质过氧化物含量明显减低。结论:在实验设定的NIV浓度范围内,NIV对培养软骨细胞有明显损伤作用,加硒有保护作用,但不能阻止损伤。
Objective: To explore the effect of Fusarium venenatina (NIV) on the metabolism of cultured chondrocytes and the protective effect of selenium. Methods: The biochemical method was used to detect the effect of NIV on the metabolism of monolayer chondrocytes. Chondrocytes were cultured in monolayer. The DNA damage of cultured monolayer chondrocytes was detected by agarose gel electrophoresis and alkaline single cell gel electrophoresis. Results: With the increase of NIV concentration in the range of NIV concentrations (0.0005 ~ 0.0200 mg / L), the matrix proteoglycan metabolism of chondrocytes was significantly reduced, the protein synthesis decreased, the DNA content decreased, the DNA damaged cells increased and the DNA damage Aggravated, the same concentration of NIV plus selenium group damage than the selenium group did not light. NIV did not affect lipid peroxidation of chondrocytes at 3 NIV concentrations, but selenium supplementation significantly reduced lipid peroxides. CONCLUSION: NIV can obviously injure cultured chondrocytes within the experimental NIV concentration range, and selenium has a protective effect but can not prevent the injury.