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目的探讨面神经损伤后,糖皮质激素受体α(GRα)在面神经核团及外周淋巴组织的表达和变化规律。方法建立新西兰兔面神经损伤模型,分别于损伤后24 h4、8 h7、2 h、7 d1、4 d取出面神经核团和颈部淋巴结,采用免疫组化SP法,观察糖皮质激素受体的表达情况。结果糖皮质激素受体α在面神经核团及外周淋巴细胞中均有表达;糖皮质激素受体α表达增高在面神经核中出现在损伤48 h后,颈部淋巴结出现在损伤72 h后。结论糖皮质激素受体α表达在面神经核团及外周淋巴细胞胞核及胞质中,在面神经受到损伤后其表达量增高,而且神经损伤较单纯外伤可以引起较长时间的局部炎症反应。
Objective To investigate the expression and alteration of glucocorticoid receptor α (GRα) in facial nucleus and peripheral lymphoid tissue after facial nerve injury. Methods The facial nerve injury model was established in New Zealand rabbits. Facial nerve nuclei and cervical lymph nodes were removed at 4 h, 2 h, 2 h, 7 h, 7 d, and 4 d after injury. Immunohistochemical SP method was used to observe the expressions of glucocorticoid receptor Happening. Results Glucocorticoid receptor α was expressed in both facial nucleus and peripheral lymphocytes. The expression of glucocorticoid receptor α increased in the facial nucleus 48 h after injury, and the cervical lymph nodes appeared at 72 h after injury. Conclusions Glucocorticoid receptor α is expressed in the nucleus and cytoplasm of facial nerve nuclei and peripheral lymphocytes. The expression of glucocorticoid receptor α is increased after the facial nerve is injured. Moreover, the nerve injury can cause a longer time local inflammatory reaction than the traumatic injury alone.