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建立同时测定当归龙荟片中芦荟苷,黄芩素,芦荟大黄素和汉黄芩素的含量的HPLC方法。采用Diamonsil C_(18)柱(200.0 mm×4.6 mm,5μm)分离,以甲醇-乙腈体积分数为0.3%磷酸溶液(220:4:200,v/v/v)为流动相进行沈脱,流速为1.0 mL/min,检测波长为225 nm。芦荟苷的线性范围为1.450～29.00μg/mL(r=0.9992),黄芩素的线性范围为0.4050～8.100μg/mL (r=0 9994),芦荟大黄素的线性范围为0.1100～2.200μg/mL(r=0.9997),汉黄芩素的线性范围为0.2160～4.320μg/mL (r=0.9991)。方法的平均回收率分别为100.7%(RSD%=0.88%),101.0%(RSD%=0.89%),100.0%(RSD=1.3%)和100.1% (RSD=1.1%)。为当归龙荟片的质量控制提供了科学的方法。
The simultaneous determination of aloin, baicalein, aloe-emodin and wogonin in Angelica Sinensis was established. The samples were separated on a Diamonsil C18 column (200.0 mm×4.6 mm, 5 μm) and eluted with a methanol-acetonitrile volume fraction of 0.3% phosphoric acid solution (220:4:200, v/v/v) as the mobile phase. The flow rate was 1.0 mL/min, detection wavelength is 225 nm. The linear range of aloin was 1.450～29.00μg/mL (r=0.9992), the linear range of baicalein was 0.4050～8.100μg/mL (r=0 9994), and the linear range of aloe-emodin was 0.1100～2.200μg/mL. (r=0.9997), the linear range of wogonin was 0.2160 to 4.320 μg/mL (r=0.9991). The average recoveries of the method were 100.7% (RSD% = 0.88%), 101.0% (RSD% = 0.89%), 100.0% (RSD = 1.3%), and 100.1% (RSD = 1.1%), respectively. Provides a scientific method for the quality control of Angelica Longhui Tablets.