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为了深入了解咖啡因对胎儿、新生儿生殖细胞的数量及其合成ＤＮＡ的影响，本实验采用低（３×１０－４ｍｏｌ／Ｌ）、中（６×１０－４ｍｏｌ／Ｌ）、高（１．２×１０－３ｍｏｌ／Ｌ）浓度咖啡因体外培养ＳＤ孕１８天胎鼠、０天及４天乳鼠睾丸组织块，培养时间分别为１周、２周、３周，观察咖啡因作用后睾丸内生殖细胞数量的变化，并且利用计算机图象分析系统测定生殖细胞ＤＮＡ的含量，以探讨两者的关系。结果如下：（１）１８天胎鼠睾丸培养组织的生殖细胞受咖啡因影响最少，０天乳鼠次之，４天乳鼠受影响较大。（２）低浓度的咖啡因对生殖细胞数量影响较少；中等浓度的咖啡因在培养３周后才有生殖细胞数量的降低；而高浓度的咖啡因在培养２周后，已有下降，３周更明显。（３）生殖细胞数量的减少往往伴随ＤＮＡ含量的降低，由此推测高浓度咖啡因长时间培养后使生殖细胞数量减少，可能通过抑制ＤＮＡ复制发挥作用。
In order to understand the effects of caffeine on fetal and neonatal germ cells and its effect on DNA synthesis, low (3 × 10-4mol / L), medium (6 × 10-4mol / L) and high 2 × 10-3mol / L) concentration of caffeine SD pregnant 18-day embryos of 18-day-old, 0 days and 4 days of testis tissue of young rats, culture time were 1 week, 2 weeks, 3 weeks, observe the role of testis after caffeine Endogenous germ cell number changes, and the use of computer image analysis system determination of germ cell DNA content, to explore the relationship between the two. The results are as follows: (1) The reproductive cells of 18-day-old fetus cultured tissue were the least influenced by caffeine, followed by 0-day-old suckling rats and 4-day-old suckling rats. (2) Low concentrations of caffeine had little effect on the number of germ cells; moderate concentrations of caffeine decreased the number of germ cells after 3 weeks of culture; while high concentrations of caffeine decreased after 2 weeks of culture, More obvious. (3) The decrease of germ cell number often accompanied with the decrease of DNA content, suggesting that the high concentration of caffeine could reduce the number of germ cells after prolonged culture, which may be through the inhibition of DNA replication.