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前列腺特异抗原(PSA)基因受雄激素调节,其雄激素应答元件(ARE)位于—170附近。ARE上游RF15序列的丢失和变异可降低雄激素对PSA基因的诱导作用。用RF15DNA片段和人前列腺肿瘤细胞LNcap或PC—3的核蛋白进行体外结合实验,结果表明RF15可与这些细胞中的某些调节蛋白结合。而且RF15对调节蛋白的结合能力受Zn~(2+)的影响。RF15可能是PSA启动子中的一个新的附属调节元件,与之结合的调节蛋白可能通过与雄激素受体的相互作用促进雄激素的诱导作用。
The prostate specific antigen (PSA) gene is upregulated by androgens and its androgen response element (ARE) is located around -170. Loss and mutation of the RF15 sequence upstream of ARE can reduce the androgen induction of PSA gene. In vitro binding experiments using RF15 DNA fragments and human prostate tumor cells LNcap or PC-3 nucleoprotein indicated that RF15 binds to some of these regulatory proteins in these cells. Moreover, the binding ability of RF15 to regulatory proteins is affected by Zn 2+. RF15 may be a new adjunct regulatory element in PSA promoter. The regulatory proteins associated with it may promote androgen induction through the interaction with androgen receptor.