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目的:观察褪黑激素对淀粉样β多肽25-35片段(Aβ_(25-35))诱导的大鼠学习记忆功能障碍的改善作用。方法:学习记忆功能检测采用大鼠跳台法、穿梭法和Moriss水迷宫法;病理组织观察采用HE染色、刚果红染色和银染色。结果:大鼠双侧海马内注射Aβ_(25-35)(20μg)可引起大鼠学习记忆功能障碍,主要表现为在跳台作业中潜伏期缩短,累计刺激时间延长,累计错误次数增多;穿梭实验中,主动回避潜伏期缩短,主动回避次数减少,被动回避次数增加;Moriss水迷宫同样也证实大鼠出现空间学习记忆障碍;HE染色发现皮质和海马神经细胞数量减少,胶质细胞呈反应性增生,嗜神经现象增多;刚果红染色血管出现阳性;银染色提示有纤维蛋白丝状物。褪黑激素(0.1,1,10mg/kg)连续给予(ig)7-10d分别对上述改变有不同程度的抑制作用。结论:褪黑激素能改善Aβ_(25-35)诱导的大鼠学习记忆功能障碍。
Objective: To observe the effect of melatonin on learning and memory dysfunction induced by amyloid β peptide 25-35 (Aβ_ (25-35)) in rats. Methods: The function of learning and memory was detected by jumping platform method, shuttle method and Moriss water maze method. The pathological tissues were observed by HE staining, Congo red staining and silver staining. Results: The bilateral hippocampal injection of Aβ 25-35 (20μg) induced learning and memory dysfunction in rats, mainly manifested as shortening the incubation period, increasing the cumulative stimulation time and increasing the total number of errors in the platform jumping operation. In the shuttle experiment , Active avoidance latency was shortened, the number of active avoidance decreased, the number of passive avoidance increased; Moriss water maze also confirmed spatial learning and memory impairment in rats; HE staining found that the number of cortical and hippocampal nerve cells decreased, glial cells were reactive hyperplasia, addicted Nerve phenomenon increased; Congo red staining of blood vessels positive; silver staining prompted fibrin filaments. Melatonin (0.1,1,10 mg / kg) continuous administration (ig) 7-10d, respectively, to varying degrees of inhibition of these changes. CONCLUSION: Melatonin can improve learning and memory dysfunction induced by Aβ 25-35 in rats.