论文部分内容阅读
目的 :研究中药复方戒毒制剂冰茶栓对海洛因依赖猴戒断症状及基因表达的影响。方法 :猕猴经海络因皮下注射 72天建立依赖模型 ;并将海洛因依赖猴共 14只 ,分 4组 ,NS组 (3只 ) ,阳性对照组 (3只 ) ,高剂量组 (4只 ) ,低剂量组 (4只 )。在冰茶栓治疗组与未经冰茶栓治疗组及正常组 ,各取猴大脑纹状体组织 ,经RNA抽提纯化 ,采用Cy5、Cy3荧光标记 ,与受体相关基因芯片杂交筛选差异表达基因。结果 :海洛因依赖猴在自然戒断时 ,前脑啡肽原基因发生上调表达 ,而经冰茶栓治疗后 ,此基因表达下调 ,白细胞抗原SB(DP)基因表达上调。结论 :海洛因依赖猴经冰茶栓治疗后 ,戒断症状明显改善 ,并造成基因表达改变。提示 :冰茶栓减轻戒断症状与恢复脑啡肽水平及提高白细胞抗原、增强免疫功能有关。
Objective : To study the effect of Xuechaan suppository, a traditional Chinese medicine compound, on the withdrawal symptoms and gene expression of heroin-dependent monkeys. METHODS: Rhesus monkeys were established by subcutaneous injection of colostrum for 72 days. A total of 14 heroin-dependent monkeys were divided into 4 groups, NS group (3), positive control group (3), and high-dose group (4). Low dose group (4). In the ice tea suppository treatment group and non-ice tea suppository treatment group and normal group, monkey brain striatum tissues were taken, purified by RNA extraction, and labeled with Cy5 and Cy3 fluorescent markers, and hybridized with receptor-associated gene chips to screen for differential expression. gene. RESULTS: The proenkephalin gene was up-regulated in heroin dependent monkeys at natural withdrawal. After treatment with ice tea suppository, the gene expression was down-regulated and the expression of leukocyte antigen SB (DP) gene was up-regulated. Conclusion : The withdrawal symptoms of heroin-dependent monkeys after treatment with ice tea suppositories were significantly improved and gene expression was changed. Tips: Ice tea suppositories to reduce withdrawal symptoms and restore enkephalin levels and increase leukocyte antigens, enhance immune function.