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目的 :研究先天性HCMV感染与胎鼠大脑皮质异常的关系。方法 :10周龄的ICR雌雄鼠按 1∶1配对 ,妊 7天时实验组行腹腔内接种HCMV ,对照组则接种HF细胞上清液。分别于妊 12、15、19天剖腹取胚胎大脑皮质进行病理学检查、病毒分离及原位杂交。结果 :实验组ICR胎鼠脑实质血管充血、出血等炎性改变 ,妊 19天胎鼠大脑皮质神经细胞变性坏死 ;3个时间点实验组病毒分离率分别为 10 %、40 %及 6 7% ,MIE和LA寡核苷酸探针原位杂交阳性率为 30 %、6 0 %、83%和 0 %、40 %、70 %。结论 :HCMV感染妊 7天ICR鼠后 ,不仅可在胎鼠大脑皮质神经细胞中复制且使大脑皮质损伤明显。
Objective: To study the relationship between congenital HCMV infection and fetal cerebral cortex abnormalities. Methods: ICR male and female 10-week-old mice were paired by 1: 1. HCMV was intraperitoneally inoculated into the experimental group at 7th day of pregnancy and the control group was inoculated with HF cell supernatant. The embryonic cerebral cortexes were harvested on day 12, day 15 and day 19 for pathological examination, virus isolation and in situ hybridization. Results: The experimental group rats ICR embryonic rat brain blood vessel congestion, bleeding and other inflammatory changes, pregnancy 19 day fetal rat cerebral cortex neurons degeneration and necrosis; three time points in the experimental group virus isolation rates were 10%, 40% and 67% The positive rates of in situ hybridization of MIE and LA oligonucleotide probes were 30%, 60%, 83% and 0%, 40% and 70%, respectively. CONCLUSION: HCMV infection in ICR rats after 7 days of pregnancy can not only replicate in cerebral cortex neurons of fetal rats but also cause obvious cerebral cortex injury.