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目的筛选野生蛇足石杉引种驯化的优质种质资源,并建立其分子鉴别方法。方法对来自福建大田、三明市郊、南靖3个不同种质资源野生蛇足石杉引种驯化,HPLC法分析不同种质资源蛇足石杉中石杉碱甲(Hup A)量;采用蛋白质双向电泳技术,分析不同种质资源蛇足石杉差异蛋白。结果种质资源来自大田的蛇足石杉适应性较强,植株长势好,成活率为65.5%,且茎叶Hup A量也较高,分别为197.80和87.54μg/g;遮阳成活率为77.8%,远高于未遮阳的成活率(7.1%);黄土地成活率为75%,远高于水田沙壤土的成活率(18.8%)。不同种质资源蛇足石杉共有15个差异蛋白质点,不同种质资源蛇足石杉的差异蛋白质点丰度差异较大。结论人工引种栽培时,土壤选择以黄土质为佳,并需要给予必要的遮阳;种质资源来自福建大田的蛇足石杉可以作为蛇足石杉优质种质资源进行引种驯化;利用蛇足石杉差异蛋白质点的丰度差异,可以作为鉴别不同蛇足石杉种质资源的依据。
Objective To screen the qualified germplasm resources introduced and domestically cultivated in Hupeus subgenilis and to establish its molecular identification method. Methods The wild Hypsizygdalus populations from three germplasms with different germplasm resources in Datian, Sanming suburbs and Nanjing of Fujian were introduced and acclimated. The contents of Hup A in different germplasm resources of Hypodermis were determined by HPLC. Two - dimensional electrophoresis Analysis of different germplasm resource Snakefoot Fusarium different proteins. Results The Germplasm Resources of Huperzia serrata from field were more adaptable, the plant grew well, the survival rate was 65.5%, and the amount of Hup A in stems and leaves was also higher, which were 197.80 and 87.54 μg / g respectively. The survival rate of shading was 77.8% (7.1%). The survival rate of loess land was 75%, far higher than the survival rate of sandy loam in paddy field (18.8%). There were 15 different protein spots in different germplasm resources of Huperzia serrata, and the difference of protein abundance of different species of Huperzia serrata differed greatly. Conclusion Artificial introduction and cultivation of loess soil is better than others, and the necessary shade must be given. Germplasm resources from the Daejeon in Fujian Province can be used as the high quality Germplasm resources of the snake shrubs. Differences in abundance of points can be used as a basis for identifying germplasm resources of Huperzia serrata.