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目的:比较空间学习记忆中孕期应激子代和对照组子代海马空间学习功能密切相关蛋白NR2B的表达。方法:建立孕期束缚应激小鼠模型,在出生后第3周用酶联免疫法检测子代雄性小鼠血清皮质酮;在出生后第9周,采用免疫蛋白印迹和real-timePCR法检测子代雄性小鼠非空间学习记忆状态及空间学习记忆中海马NR2B的表达水平。结果:(1)基础状态下孕期应激子代血清皮质酮水平与对照组子代并无差异,应激状态下孕期应激子代血清皮质酮水平(43.7×104pg/ml)明显高于对照组子代(18.7×104pg/ml);(2)孕期应激雄性子代在出生后第9周海马NR2B mRNA和蛋白表达均显著低于对照组雄性子代。但经过空间学习记忆训练,两组海马NR2B蛋白水平无明显差异。结论:孕期应激减少子代海马神经元NR2B的表达,但水迷宫行为训练可逆转孕期应激造成的子代海马神经元NR2B的表达降低。
OBJECTIVE: To compare the expression of NR2B, a closely related spatial learning function in the offspring of stress-induced offspring of pregnant and pregnant offspring in spatial learning and memory. Methods: The mouse model of restraint stress during pregnancy was established. Serum corticosterone of offspring male mice was detected by enzyme-linked immunosorbent assay (ELISA) in the third week after birth. At week 9 postnatal, immunoblotting and real-time PCR The level of NR2B expression in nonspecific learning and memory and spatial learning and memory in the male mice. Results: (1) There was no difference in serum corticosterone level between pre-term and post-pregnancy progeny in pregnancy compared with progeny in control group. The level of serum corticosterone (43.7 × 104 pg / ml) in progeny during pregnancy was significantly higher than that in control (18.7 × 104 pg / ml). (2) The expression of NR2B mRNA and protein in hippocampus of pregnant male offspring at 9 weeks after birth was significantly lower than that of male offspring in control group. However, after spatial learning and memory training, there was no significant difference in NR2B protein levels between the two groups. CONCLUSIONS: Stress during pregnancy reduces NR2B expression in hippocampal neurons, but water maze behavioral training can reverse the decrease of NR2B expression in the offspring hippocampus during pregnancy.