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目的:检测骨桥蛋白(Osteopontin,OPN)在口腔扁平苔藓(Orallichen planus,OLP)局部粘膜和外周血中的表达水平,探索其在OLP免疫发病机制中的作用。方法:免疫组化法检测OPN在局部粘膜组织的表达;ELISA检测外周血清OPN和白细胞介素12(Interleukin-12,IL-12)的蛋白浓度;分离正常人外周血单个核细胞(Peripheral blood mononuclear cells,PBMCs),分OPN刺激组和对照组,体外活化72小时,流式细胞学检测淋巴细胞的凋亡率。结果:OPN在OLP患者局部病变粘膜组织的表达较健康对照组明显增强(P<0.01);OPN和IL-12在OLP患者血清中的浓度均明显高于健康对照组(P<0.01),而且二者存在显著正相关(r2=0.6585,P<0.0001);体外活化淋巴细胞的凋亡率,OPN刺激组明显低于对照组(P<0.05)。结论:OPN在OLP局部粘膜和外周血中的表达水平明显升高,并且可以抑制体外活化淋巴细胞的凋亡,提示OPN在OLP的发病发展中可能起着重要的促进作用。
OBJECTIVE: To detect the expression of osteopontin (OPN) in the local mucosa and peripheral blood of oral lichen planus (OLP) and to explore its role in the pathogenesis of OLP. Methods: Immunohistochemistry was used to detect the expression of OPN in local mucosa. The protein levels of OPN and IL-12 in peripheral blood were detected by ELISA. Peripheral blood mononuclear cells Cells and PBMCs were divided into OPN stimulation group and control group. The cells were activated in vitro for 72 hours. The apoptosis rate of lymphocytes was detected by flow cytometry. Results: The expression of OPN in OLP patients was significantly higher than that in healthy controls (P <0.01). The serum levels of OPN and IL-12 in OLP patients were significantly higher than those in healthy controls (P <0.01) There was a significant positive correlation between them (r2 = 0.6585, P <0.0001). The apoptosis rate of activated lymphocytes in vitro was significantly lower than that of the control group (P <0.05). CONCLUSION: The expression of OPN in OLP mucosa and peripheral blood is significantly increased, and it can inhibit the apoptosis of activated lymphocytes in vitro, suggesting that OPN may play an important role in the pathogenesis of OLP.