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花生(Arachis hypogaea L.)高油酸性状受2对同源非等位隐性基因ol1和ol2调控,分别编码Ah FAD2A和Ah FAD2B。本研究利用红外无损检测技术分析不同花生品种的油酸、亚油酸及粗脂肪含量,并设计等位基因特异引物,对不同油亚比值的12个花生品种进行AS-PCR检测分析。结果表明:对12个花生基因型均能准确分析出Ah FAD2基因型,其中1514、606、9102、614和1474的基因型为OL1OL1OL2OL2,其相应的O/L值位于0.971~1.759;花17、1513、1476和1586的基因型是ol1ol1/OL2OL2,其相应的O/L值位于2.252~3.679;1504、1505和1515的基因型为ol1ol1ol2ol2,其相应的O/L值位于8.204~12.79。综合12个花生品系籽粒O/L值和Ah FAD2基因型的相关性结果表明,基因型的改变直接导致O/L值的变化,但与含油量无特定相关性。
The high oleic trait in peanut (Arachis hypogaea L.) was regulated by two pairs of homologous non-allelic recessive genes, ol1 and ol2, encoding Ah FAD2A and Ah FAD2B, respectively. In this study, the content of oleic acid, linoleic acid and crude fat in different peanut varieties were analyzed by using non-destructive infrared spectroscopy. Allele-specific primers were designed for the analysis of 12 peanut varieties with different oil-sub-ratio. The results showed that Ah FAD2 genotype could be accurately analyzed for all 12 peanut genotypes. The genotypes of 1514, 606, 9102, 614 and 1474 were OL1OL1OL2OL2 with corresponding O / L values ranging from 0.971 to 1.759. The genotypes of 1513, 1476 and 1586 were ol1ol1 / OL2OL2 with corresponding O / L values ranging from 2.252 to 3.679. The genotypes of 1504, 1505 and 1515 were olol1ol2ol2 with corresponding O / L values ranging from 8.204 to 12.79. Correlation analysis of grain O / L value and Ah FAD2 genotype in 12 peanut varieties showed that the change of genotype led to the change of O / L value directly but had no specific correlation with oil content.