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目的 探讨 ｐ１５、ｐ１６基因启动子区 ＣＰＧ岛甲基化在多发性骨髓瘤发病中的作用。方 法 利用甲基化特异性 ＰＣＲ技术，研究骨髓瘤细胞株（Ｕ２６６、ＬＰ１、ＫＭ３）及多发性骨髓瘤患者 ｐ１５、ｐ１６基因甲基化状态。结果 Ｕ２６６、ＬＰ１细胞 ｐ１５、ｐ１６基因处于完全甲基化状态；ＫＭ３细胞 则为完全未甲基化模式；ＭＭ患者的ｐ１５、ｐ１６基因启动子区甲基化比例分别为 ５２．３８％及 ５７．１４％。 ｐ１５、ｐ１６基因甲基化与 ＭＭ分期无关（Ｐ＞０．０５）。结论 ｐ１５、ｐ１６基因甲基化在多发性骨髓瘤中 较为常见，这可能为多发性骨髓瘤的治疗提供借鉴。
Objective To investigate the role of CPG island methylation in the promoter region of p15 and p16 genes in the pathogenesis of multiple myeloma. Methods The methylation status of p15 and p16 gene in myeloma cell lines (U266, LP1, KM3) and multiple myeloma was studied by methylation-specific PCR. Results The p15 and p16 genes in U266 and LP1 cells were completely methylated while KM3 cells were completely unmethylated. The methylation rates of p15 and p16 genes in MM patients were 52.38% and 57%, respectively. 14%. Methylation of p15 and p16 genes had no correlation with MM stage (P> 0.05). Conclusion The methylation of p15 and p16 genes is more common in multiple myeloma, which may provide reference for the treatment of multiple myeloma.