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作者研究目的是寻找确定卵巢癌中涉及P53基因特殊密码子和突变类型的突变频谱。同时也检测了P53基因突变肿瘤中,P53等位基因缺失率。 组织标本取自经组织学检查,确定样本肿瘤细胞含量超过75％的63例手术切除的卵巢癌。分别从癌标本及配对的非癌组织区域或白细胞中提取总RNA。提纯RNA经逆转录产生P53基因4-10外显子的cDNA拷贝。实验及对照组提取物分别经PCR反应以产生足够的模版供DNA序列分析。每扩增DNA模板至少有两个独立产物存在双脱氧序列为
The aim of the study was to find out the mutation frequency spectrum for determining the specific codon and mutation type of P53 gene in ovarian cancer. Also detected P53 gene mutation in the tumor, P53 allele deletion rate. Tissue samples were taken from 63 surgically excised ovarian cancers that were histologically examined to determine that the sample contained more than 75% of the tumor cells. Total RNA was extracted from cancer specimens and paired non-cancerous tissue areas or leukocytes, respectively. Purified RNA was reverse transcribed to generate a cDNA copy of exon 4-10 of P53 gene. The experiment and control group extracts were respectively subjected to PCR reaction to generate sufficient templates for DNA sequence analysis. There are at least two separate products for each DNA template amplification dideoxy sequence