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应用抗人nm23基因产物／NDPK的多克隆抗体，研究小鼠肝脏癌前病变中un23基因的表达状况。津白2纯系小白鼠随机分成三组：A组、B组和对照组。黄曲霉素Bl容于二甲基亚砜，以5μg／日·只量拌于饲料投食A和B组小鼠，B组另以烟丝30g／日重烟，经半年实验，取肝脏切片行免疫组织化学染色。使用医学图像处理系统检测nm23／NDPK表达强度。结果：三组小鼠肝脏nm23／NDPK表达强度呈A组＞B组＞对照组的趋势，与肝细胞核DNA含量的变化趋势相同，各组间差异均具有显著性意义（P＜0．05）。我们推断：黄曲霉素B1诱导的小鼠肝脏癌前病变中，nm23基因产物／NDPK表达上调可能与细胞增殖有关。
The polyclonal antibody against human nm23 gene product/NDPK was used to study the expression of un23 gene in mouse precancerous liver. Jinbai 2 pure white mice were randomly divided into three groups: A group, B group and control group. Aflatoxin B1 was contained in dimethyl sulfoxide, and the mice were fed with A/B in a dose of 5 μg/day. The B group was treated with 30 g/day heavy tobacco and the liver samples were taken after half a year of experiment. Immunohistochemical staining. The intensity of nm23/NDPK expression was detected using a medical image processing system. RESULTS: The expression of nm23/NDPK in the liver of the three groups showed the trend of group A> group B> control group, and the change trend of nuclear DNA content of hepatocytes was the same, with significant differences among groups (P<0.05). . We conclude that the upregulation of nm23 gene product/NDPK expression in mouse liver precancerous lesions induced by aflatoxin B1 may be related to cell proliferation.