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目的:研究微囊化新生猪胰岛细胞异种移植影响生物相容性的因素以及对1型糖尿病的疗效。方法:注射链脲霉素诱发糖尿病大鼠模型后,将不同组微囊化新生猪胰岛细胞、未微囊化新生猪胰岛细胞及空微囊,经腹腔植入1型糖尿病大鼠体内,移植后不应用任何免疫抑制剂,测量移植后大鼠血糖、体重、胰岛素C肽的变化。结果:移植后6~8周实验组、对照组3糖尿病大鼠血糖、体重逐渐恢复正常水平;移植后实验组、对照组1、2、3血清C肽水平升高,对葡萄糖刺激实验反应明显;胰岛素释放试验显示葡萄糖可刺激胰岛细胞释放胰岛素实验组与对照组比较有统计学意义(p<0.01)。结论:微囊周围细胞过度增生是导致移植物功能丧失的主要原因;微囊化新生猪胰岛细胞可以纠正糖尿病大鼠高血糖状态,在体内可以生长、分化,并增加胰岛素分泌水平。
OBJECTIVE: To study the factors affecting the biocompatibility of microencapsulated neonatal porcine islet xenotransplantation and its effect on type 1 diabetes mellitus. Methods: After injecting streptozotocin-induced diabetic rat model, different groups of microencapsulated neonatal porcine islet cells, non-microencapsulated neonatal porcine islet cells and empty microcapsules were implanted intraperitoneally into type 1 diabetic rats and transplanted After the application of any immunosuppressive agent, the blood glucose, body weight and insulin C peptide level in the rats after transplantation were measured. Results: After 6 to 8 weeks, blood glucose and body weight of diabetic rats in experimental group and control group gradually recovered to normal levels. Serum C-peptide levels increased in experimental group and control group 1, 2 and 3 after transplantation, and the response to glucose stimulation test was obvious ; Insulin release test showed that insulin can be stimulated by glucose in islet cells release experimental group compared with the control group was statistically significant (p <0.01). CONCLUSION: Hyperplasia of cells around the microcapsule is the main reason leading to the loss of graft function. Microencapsulated neonatal porcine islet cells can correct the hyperglycemia in diabetic rats, and can grow and differentiate in vivo and increase the level of insulin secretion.