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目的通过观察实验性铅性肾病大鼠肾脏纤维连接蛋白(FN)的表达及血清中γ-干扰素(IFN-γ)的水平,探讨铅性肾病肾脏纤维化的发病机制。方法SD大鼠48只适应性饲养1周,随机分为6组,每组8只,A、B、C组为染铅组,分别给予质量分数为0.5%的醋酸铅喂饮1、2、3个月;Ac、Bc、Cc组为相应正常对照组,分别给予蒸馏水喂饮1、2、3个月。实验结束时称。肾重、体重变化,原子吸收光谱石墨炉法测定血铅、肾铅含量,电子显微镜观察肾脏的超微病理改变,免疫组化和RT-PCR方法检测。肾组织中FN蛋白及其mRNA的表达,ELISA方法检测血清中IFN-γ含量。结果染铅3个月时大鼠体重明显低于对照组,肾重明显高于对照组,肾脏在电镜下出现超微病理改变;染铅2个月后,肾组织中FN蛋白及其mRNA的表达明显增高;3个染铅组与相应对照组比较,血铅、肾铅含量明显增高;血清中IFN-γ的含量则明显降低。结论染铅2个月后肾组织即启动了纤维化的早期环节,铅导致的肾脏纤维化与IFN-γ的降低有关。
Objective To investigate the pathogenesis of renal fibrosis in lead nephropathy by observing the expression of renal fibronectin (FN) and the level of interferon-γ (IFN-γ) in experimental lead nephropathy rats. METHODS: Forty-eight SD rats were housed for 1 week and were randomly divided into 6 groups with 8 rats in each group. Group A, B and C were lead-exposed groups, which were given lead acetate 0.5% Month; Ac, Bc, Cc group for the corresponding normal control group, respectively, given distilled water feeding 1,2,3 months. The end of the experiment said. Kidney weight and body weight were measured. The content of lead and lead in blood and lead were determined by graphite furnace atomic absorption spectrometry. The ultrastructural changes of kidneys were observed by electron microscopy. Immunohistochemistry and RT-PCR were used to detect the pathological changes. The expression of FN protein and its mRNA in renal tissue and the content of IFN-γ in serum were measured by ELISA. Results At 3 months after the lead exposure, the body weight of rats was significantly lower than that of the control group, the kidney weight was significantly higher than that of the control group, and the ultrastructure of the kidney was observed under electron microscope. After 2 months of lead exposure, the expression of FN protein and its mRNA Compared with the corresponding control group, the levels of lead in blood lead and kidney were significantly increased, and the content of IFN-γ in serum was significantly decreased. Conclusion Renal tissue activated the early stage of fibrosis after lead exposure for 2 months. Lead-induced renal fibrosis was associated with a decrease in IFN-γ.