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目的:研究小干扰RNA（siRNA）干扰高迁移率族蛋白B1（HMGB1）对人喉鳞状细胞癌Hep-2/v耐药细胞株生物学特性的影响。方法:将siRNA-HMGB1转染至Hep-2/v细胞并设为阳性对照组，同时设转染随机序列的阴性对照组和无转染的空白对照组，采用Western blot检测HMGB1和MDR1蛋白量的表达变化，采用CCK-8检测细胞增殖活性变化，采用流式细胞术检测细胞周期变化，采用细胞免疫荧光检测HMGB1和MDR1蛋白的表达变化。结果:与阴性对照组和空白对照组相比，阳性对照组细胞增殖能力有所下降，瞬时转染后24 h时下降20.18%（n P<0.01），48 h下降21.92%（n P<0.05）；Gn 1期细胞比例明显增加；HMGB1蛋白表达量下降41.38%（n P<0.01），MDR1蛋白表达下降26.77%（n P<0.05）。n 结论:抑制HMGB1在Hep-2/v细胞内的表达，可以抑制其细胞增殖，降低其细胞的耐药性。“,”Objective:To study the effect of small interfering RNA (siRNA) silencing high mobility group box 1 (HMGB1) on the biological characteristics of hep-2/v resistant cells line of human laryngeal squamous cell carcinoma (HSCC).Methods:In Dalian Municipal Central Hospital, siRNA-HMGB1 was transfected into Hep-2/v cells. The expression of HMGB1 and MDR1 protein were detected by Western blot and cell immunofluorescence, cell proliferation activity was detected by CCK-8, and cell cycle was detected by flow cytometry.Results:Compared with that of the negative control group and the blank control group, the proliferation ability of the positive control group decreased by 20.18% at 24 h (n P<0.01) and 21.92% at 48 h (n P<0.05). The proportion of Gn 1 cells increased obviously. The expression of HMGB1 protein decreased 41.38% (n P<0.01), the expression of HMGB1 protein decreased 26.77% (n P<0.05).n Conclusions:Reduction of HMGB1 expression in hep-2/v cells can inhibit cell proliferation and reduce drug resistance.