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采用敏感的ＭＴＴ法测定了嗜麦芽假单胞菌转化黑色素的抗流感病毒作用。测定结果表明：纯化的黑色素毒性极低，对ＭＤＣＫ细胞的无毒界限为０．２ｍｇ／ｍｌ，远高于其有效的作用浓度；１０～２０μｇ／ｍｌ的黑色素能有效抑制流感病毒（血凝效价１∶３２）致细胞病变。病毒感染７２ｈ后用ＭＴＴ法测定，其宿主ＭＤＣＫ细胞保护百分率达９５％以上；此外，加黑色素后的ＭＤＣＫ细胞，抗流感病毒感染的能力优于目前抗病毒的有效药物病毒唑，前者的最佳作用浓度（２０μｇ／ｍｌ）比后者（１００μｇ／ｍｌ）低５倍。可以认为嗜麦芽假单胞菌转化黑色素具有毒性低、抗流感病毒能力强的特点
The sensitive MTT method was used to determine the anti-influenza virus activity of Pseudomonas maltophilia transformed with melanin. The results showed that the purified melanin had very low toxicity and its non-toxic limit to MDCK cells was 0.2mg / ml, which was much higher than its effective concentration. Melanin with 10 ~ 20μg / ml could effectively inhibit influenza virus Valence 1:32) cytopathic effect. After 72h virus infection, the percentage of MDCK cells in the host cells was over 95% as determined by MTT assay. In addition, MDCK cells with melanin showed better anti-influenza virus infection than the current antiviral drug ribavirin, the former being the best The effect concentration (20μg / ml) is 5 times lower than the latter (100μg / ml). It can be considered that Pseudomonas maltophilia transformed melanin has the characteristics of low toxicity and strong anti-influenza virus