澳洲龙纹斑雌、雄亲鱼营养组成比较分析

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目的探索澳洲龙纹斑亲鱼生长关键阶段的营养组成,提升澳洲龙纹斑人工繁殖技术,以增加其鱼类苗种资源。方法通过分析化学方法检测澳洲龙纹斑雌、雄亲鱼的蛋白质、脂肪、氨基酸和脂肪酸等含量,并进行分析和评价。结果澳洲龙纹斑雌、雄亲鱼肌肉中脂肪和蛋白质含量都呈极显著差异,雌、雄亲鱼肌肉中17种氨基酸除了组氨酸和胱氨酸的含量无显著性差异(P>0.05)外,其他15种氨基酸均为雌亲鱼含量极显著高于雄亲鱼(P<0.01),雌亲鱼的氨基酸总量、必需氨基酸总量、非必需氨基酸总量、甜味氨基酸总量、苦味氨基酸总量、酸味氨基酸总量、呈味氨基酸总量、含硫氨基酸总量、支链氨基酸总量和芳香族氨基酸总量等均极显著高于雄亲鱼(P<0.01)。澳洲龙纹斑雌、雄亲鱼脂肪酸组成中含量最高的均是油酸C18∶1n9c,棕榈酸C16∶0次之,而且雌亲鱼的棕榈酸C16∶0含量极显著高于雄亲鱼(P<0.01),但雌、雄亲鱼的油酸C18∶1n9c含量无显著性差异,雌、雄亲鱼n-3/n-6的比值都为1.0。澳洲龙纹斑雌、雄亲鱼都达到了WHO/FAO提出的E/T应为40%左右和E/NT应为60%以上的参考蛋白质模式标准,雌、雄亲鱼的氨基酸比值系数分SRCAA相近且高达78%,而且富含不饱和脂肪(∑UFA为57.4%和55.4%)。结论澳洲龙纹斑雌、雄亲鱼的主要营养成分脂肪、蛋白质差异显著。建议澳洲龙纹斑雌、雄亲鱼分池饲养,饲喂不同营养的饲料。雌、雄亲鱼肌肉营养价值高,是优质的动物蛋白源。“,”Objective Nutritional compositions in the meat of female and male Murray cods (Maccullochella peelii peelii) at critical growth stage were determined for feed improvement of the aquaculture. Method Contents of protein, amino acids (AAs), fat, and fatty acids (FAs) in the filets were analyzed for the evaluation. Result Significant differences were found on fat and protein contents between the female and male fish fillets. Among 17 AAs, there was no significant difference between the females and males on cystine (Cys) or histidine (His) (P>0.05). However, the females had extremely significantly higher contents of the other AAs than the males (P<0.01). The total AA as well as the essential, non-essential, sweet, bitter, acidic, flavoring, sulfur, branch-chain, and aromatic AAs were extremely significantly higher in the females than the males (P<0.01). The highest content of FAs in the fish filets was C18:1n9c, followed by C16:0. The C16:0 FA in meat was extremely significantly higher for the female than the male (P<0.01), but no significant difference on C18:1n9c. The n-3/n-6 ratios were same for the female and male at 1.0. The E/T of both female and male cods surpassed the reference protein standard at 40%, and the E/NT at more than 60%, as proposed by WHO/FAO. The SRCAA was 78% for both male and female Murray cods. The filets were rich in unsaturated FAs, i.e., 57.4% for the females and 55.4% for the males. ConclusionThe major nutritional components in the filets of the male and female parental Murray cods were significantly different, indicating that they ought to be fed with differently formulated forages in separate ponds. Nutritionally, the meat of either female or male Murray cods was highly recommended as a source of animal protein.
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