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弯曲菌肠炎主要是由空肠弯曲菌引起的一种以腹泻为主要症状的疾病。我们于1985年11月至1986年10月,从497例急性腹泻病人粪便中分得26株空肠弯曲菌。现报告如下: 方法与步骤 1.病例选择:凡腹泻3次以上/日(含3次)初诊患者,由肠道专科门诊医生统一登记,同时把粪便作常规及细菌学检查。 2.标本处理:将患者新鲜粪便,直接或用Cary—Blair运送培养基,接种弯曲菌培养基(上海市卫生防疫站提供),以微需氧袋,42℃48小时孵育后,选取可疑菌落进行形态学及初步生化检定,菌株保存于FMPG培养基地,最后作系统生化及生物分型。同时做沙门氏、志贺氏、大肠杆菌及弧菌等分离。药敏纸片由上海市医学化验所提供。
Campylobacter enteritis is mainly caused by Campylobacter jejuni with diarrhea as the main symptom of the disease. From November 1985 to October 1986, we identified 26 strains of Campylobacter jejuni from the stools of 497 acute diarrhea patients. The report is as follows: Methods and Procedures 1. Case Selection: Patients who have newly diagnosed diarrhea more than 3 times / day (including 3 times) are enrolled by intestine specialist outpatients, and routine and bacteriological examination of excrement. 2. Specimen treatment: The patients were freshly stool, directly or with Cary-Blair transport medium, inoculated with Campylobacter medium (Shanghai Health and Epidemic Prevention Station provided) to micro-aerobic bags, 42 ℃ 48 hours after incubation, select the suspected colonies Morphology and preliminary biochemical tests were performed. The strains were stored in FMPG medium and finally biosynthesized and biologically characterized. At the same time to do Salmonella, Shigella, Escherichia coli and Vibrio separation. Drug susceptibility paper provided by the Shanghai Medical Laboratory.