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目的 比较苦参碱与E 4 0 31,多非利特和RP5 886 6对家兔单个心室肌细胞的内向整流钾电流(Ik1)的效价和效能的不同 ,揭示苦参碱抗心律失常作用弱于西药的原因。方法 应用全细胞膜片钳技术记录苦参碱与E 4 0 31,多非利特和RP5 886 6对家兔Ik1的影响。结果 苦参碱 1和 10 μmol·L- 1对家兔Ik1无明显影响。在实验电压为 - 12 0mV和保持电压为 - 70mV ,苦参碱 5 0和 10 0 μmol·L- 1对Ik1分别抑制达 6 % (n =8,P <0 .0 5 )和 8% (n =8,P <0 .0 5 ) ;在实验电压为 - 5 0mV ,抑制Ik1达 4 % (n =8,P <0 .0 5 )和 8% (n =8,P <0 .0 5 )。在实验电压为 - 12 0mV ,E 4 0 311和 10 μmol·L- 1使Ik1分别降低10 % (n =6 ,P <0 .0 5 )和 4 5 % (n =6 ,P <0 .0 5 )。在 - 5 0mV ,Ik1分别降低 5 % (n =6 ,P <0 .0 5 )和35 % (n =6 ,P <0 .0 5 )。在实验电压为 - 12 0mV ,多非利特 1和 10 μmol·L- 1使Ik1降低 19% (n =8,P <0 .0 5 )及 2 5 % (n =8,P <0 .0 5 )。在 - 5 0mV ,Ik1分别降低 11%和 19% (n =8,P <0 .0 5 )。在实验电压为 - 12 0mV ,RP5 886 6 1和 10 μmol·L- 1使Ik1分别降低 2 1% (n =8,P <0 .0 5 )和 5 0 % (n =8,P <0 .0 5 )。在 - 5 0mV ,Ik1分别降低 6 % (n =8,P <0 .0 5 )和11% (n =8,P <0 .0 5
Objective To compare the potency and potency of matrine with E 4 0 31, dofetilide and RP 5 886 6 on inward rectifier potassium currents (Ik1) in single rabbit ventricular myocytes, and to elucidate the antiarrhythmic effects of matrine Weaker than Western medicine reasons. Methods Whole cell patch clamp technique was used to record the effect of matrine and E4031, dofetilide and RP5886 6 on rabbit Ik1. Results Matrine 1 and 10 μmol·L-1 had no significant effect on Ik1 in rabbits. Matrine 50 and 10 0 μmol·L -1 inhibited Ik1 by 6% (n = 8, P <0.05) and 8% (n = 8) respectively at an experimental voltage of -120 mV and a holding voltage of -70 mV (n = 8, P <0. 05), and the inhibition of Ik1 was 4% (n = 8, P <0.05) and 8% 5). The Ik1 level was reduced by 10% (n = 6, P <0.05) and 45% (n = 6, P <0) at the experimental voltage of -120 mV, E 4 0 311 and 10 μmol·L -1, respectively. 0 5). At -50 mV, Ik1 decreased 5% (n = 6, P <0.05) and 35% (n = 6, P <0.05), respectively. At an experimental voltage of -120 mV, dkF1 and 10 μmol·L-1 decreased Ik1 by 19% (n = 8, P <0.05) and 25% (n = 8, P < 0 5). At -50 mV, Ik1 decreased by 11% and 19%, respectively (n = 8, P <0.05). When Ik1 was decreased by 21% (n = 8, P <0.05) and 50% (n = 8, P <0) at the experimental voltage of -120 mV and RP5 886 6 1 and 10 μmol·L -1, .0 5). At -50 mV, Ik1 decreased by 6% (n = 8, P <0.05) and 11% (n = 8, P <0.05