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为研究杂交过程中精卵的结合以及相关生物学特征,作者利用扫描电镜观察了云纹石斑鱼(Epinephelus moara)卵子形态结构以及云纹石斑鱼♀×七带石斑鱼(E.septemfasciatus)♂杂交时的精子入卵过程。结果发现,云纹石斑鱼(♀)×七带石斑鱼(♂)杂交与亲本自交的精子入卵过程没有本质的区别。云纹石斑鱼卵表面布满纵横交错、走向不确定的网纹,在受精孔区可观察到前庭、受精孔和微小孔。成熟的精卵相遇时相互激活,产生一系列胞间反应。受精后0~25 s,可观察到精子附着在卵子受精孔周围;受精30~45 s,已有精子进入卵子,并且明显可见精子尾部留在受精孔外;精子入卵后,受精孔内有受精锥形成,周围可观察到絮状的受精塞,将受精孔封闭;授精90 s~3 min,卵表面处于修复状态。整个过程未发现有多精入卵的现象。
In order to study the combination of sperm and egg during hybridization and related biological characteristics, the author observed the morphological structure of the eggs of Epinephelus moara by scanning electron microscopy and the morphological changes of E. japonicus (E.septemfasciatus) ) ♂ hybrid sperm into the egg process. The results showed that there was no essential difference in the process of egg into the egg between the hybrid grouper (♀) × seven grouper (♂) and the parental selfing. Moire grouper egg surface covered with criss-cross, to the uncertainty of the texture, in the fertilization hole area can be observed vestibular, fertilization holes and tiny holes. Mature sperm eggs interact with each other to produce a series of intercellular reactions. 0 ~ 25 s after fertilization, sperm can be observed around the fertilized egg oozing; fertilization 30 ~ 45 s, sperm into the egg, and clearly visible sperm tail left in the fertilization hole; sperm into the egg, fertilization hole The coning cone formed around the flocculent plug can be observed, the fertilization hole closed; insemination 90 s ~ 3 min, the egg surface is in repair. The whole process did not find the phenomenon of more eggs.