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以盆栽香橙为试材,分析不同施用浓度Se~(6+)和Se~(4+)对植株生长和抗坏血酸(As A)-谷胱甘肽(GSH)循环的影响.结果表明:两种价态硒均可促进香橙生长,主要表现在增加了叶面积、株高、鲜质量和干质量.施用Se~(6+)显著提高了香橙硒含量,且硒主要分配在叶片;而施用Se~(4+)虽能提高硒含量,但其含量远低于Se~(6+)处理,且主要分配在根系.施硒提高了叶片叶绿素和过氧化氢(H2O2)含量,且Se~(6+)处理高于Se~(4+)处理.Se~(6+)浓度≤2.0 mg·L-1处理能提高谷胱甘肽还原酶(GR)与谷胱甘肽过氧化物酶(GPX)活性、GSH与氧化型谷胱甘肽(GSSG)量,Se~(6+)浓度≥4.0 mg·L-1处理降低GSH循环的物质和酶活性;而Se~(4+)浓度≤2.0 mg·L-1处理能提高脱氢抗坏血酸还原酶(DHAR)与抗坏血酸过氧化物酶(APX)活性,且具有较高的As A/[As A+脱氢抗坏血酸(DHA)]比值,Se~(4+)浓度≥4.0 mg·L-1处理的GSH循环的物质和酶活性升高.综上,硒的不同价态和施用浓度对As A-GSH循环相关物质含量和酶活性的影响不同.结合香橙生长指标和抗氧化水平,Se~(6+)和Se~(4+)的适宜浓度分别为2.0和4.0 mg·L~(-1).
The effects of different concentrations of Se ~ (6 +) and Se ~ (4 +) on the growth of plants and the As A - GSH cycle were studied using potted orange as test material.The results showed that: The price of selenium could promote the growth of orange, which mainly increased the leaf area, plant height, fresh weight and dry weight. Se (6+) significantly increased selenium content of orange, and selenium was mainly distributed in leaves. However, the application of Se ~ (4 +) increased selenium content, but its content was much lower than that of Se ~ (6+), and was mainly distributed in the root system.Selenium application increased the contents of chlorophyll and hydrogen peroxide (H2O2) Se ~ (6+) treatment was higher than that of Se ~ (4+) treatment.Se ~ (6 +) concentration ≤2.0 mg · L-1 treatment could increase glutathione reductase (GR) and glutathione peroxidation GPX, GSH, GSSG and Se ~ (6 +) ≥4.0 mg · L-1 could reduce the activity of GSH cycle substances and enzymes, while Se ~ (4+ ) Concentration of 2.0 mg · L-1 could increase the activity of DHAR and APX, and had a higher As A / As A ratio of dehydroascorbic acid (DHA) , And the activities of GSH cycle substance and enzyme in Se ~ (4 +)> 4.0 mg · L-1 increased.In summary, The effects of different valences and application concentrations on the contents of As A-GSH-related substances and the activity of enzymes were different.Combined with the growth index and antioxidant activity of As orange, the suitable concentrations of Se ~ (6+) and Se ~ (4+) 2.0 and 4.0 mg · L -1.