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为了解长期缺氧患者TXB_2、6-KPGF_(1α)水平,选取了各类患者164名,分别作两者测定,并进行统计与分析,现报告如下。 对象和方法 一、对象: (一)正常组:国税局体检人员60名,休息20分钟后抽取静脉血; (二)缺氧组:共164例(各类气管炎、支气管炎、肺部疾病患者,以及其它继发性缺氧患者)。 二、方法: (一)标本:抽取静脉血1.8ml,用2%EDTA-Na_2 1:9抗凝,立即离心分离血浆,-20℃冻存。 (二)试剂:TXB_2、6-KPGF_(1α)由苏州医学院血栓与止血研究室提供。 (三)方法:放射免疫测定法(RIA),曲线拟合方
To understand the long-term hypoxia TXB_2, 6-KPGF_ (1α) levels, select 164 patients of various types, were measured for both, and statistics and analysis, are as follows. Subjects and Methods 1. Subjects: (1) Normal group: 60 people were examined by the IRS, and venous blood was drawn after 20 minutes of rest; (2) 164 cases of hypoxia group (all types of bronchitis, bronchitis, pulmonary diseases Patients, and other secondary hypoxic patients). Second, the method: (a) specimens: 1.8ml venous blood drawn, with 2% EDTA-Na_2 1: 9 anticoagulant, immediately centrifuged plasma, frozen at -20 ℃. (B) Reagents: TXB_2, 6-KPGF_ (1α) provided by the Suzhou Medical College Thrombosis and Hemostasis Laboratory. (Three) methods: radioimmunoassay (RIA), curve fitting side