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目的 :对照比较胶体金法与酶联免疫法对艾滋病抗体的检出效果。方法 :回顾性分析2016年2月~2017年2月自公安机关羁钾人群、娱乐场所性服务者等高危人群与临床术前检查、产前检查及其自愿咨询者中获取的376份血清资料,分别采用血清酶联免疫吸附法与胶体金法检测本组对象血清中艾滋病抗体。结果 :酶联免疫法检测艾滋病抗体假阳性率明显低于胶体金法,二者差异具备统计学意义(P<0.05)。结论 :艾滋病抗体检测中酶联免疫吸附法检测准确性优于胶体金法,对于有条件的实验室或采供血机构前一种检测方法尤为适用。对于边远地区基层医院,检测耗时短且操作简单的胶体金法更适用。
Objective: To compare the detection of AIDS antibody by colloidal gold method and enzyme-linked immunosorbent assay. Methods: A retrospective analysis was conducted on 376 serum samples obtained from pre-operative examinations and voluntary counselors from high-risk groups such as potassium poisoning and entertainment services in public security organs from February 2016 to February 2017 , Respectively, using serum enzyme-linked immunosorbent assay and colloidal gold method to detect the serum antibodies in this group of AIDS. Results: The false positive rate of HIV antibody detection by enzyme-linked immunosorbent assay was significantly lower than that by colloidal gold method, with statistical significance (P <0.05). Conclusion: The detection accuracy of ELISA is superior to that of colloidal gold assay, which is especially applicable to the former detection method of conditional laboratory or blood collection and delivery institutions. For the grass-roots hospitals in remote areas, detection time-consuming and simple gel colloidal gold method is more applicable.