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目的检测子宫肌瘤及瘤旁正常平滑肌组织中microRNA-18a(miR-18a)及雌激素受体α(ERα)的表达差异,探讨microRNA-18a及ERα与子宫肌瘤的关系。方法用原位杂交法和免疫组化法分别检测microRNA-18a和ERα在子宫肌瘤及瘤旁正常平滑肌组织石蜡切片中的表达,并评价microRNA-18a及ERα的表达之间的关系。结果子宫肌瘤组织中microRNA-18a表达显著低于瘤旁正常平滑肌组织(P<0.05),而ERα表达显著高于瘤旁正常平滑肌组织(P<0.05)。子宫肌瘤组织及瘤旁正常平滑肌组织中microRNA-18a及ERα的表达量呈负相关。结论 microRNA-18a可能通过影响ERα在子宫肌瘤中的表达,从而在子宫肌瘤发生发展过程中起到重大作用;microRNA-18a可能成为治疗子宫肌瘤的新靶标。
Objective To detect the expression of microRNA-18a (miR-18a) and estrogen receptor alpha (ERα) in uterine leiomyoma and normal para-tumor tissue, and to explore the relationship between microRNA-18a, ERα and uterine leiomyoma. Methods The expression of microRNA-18a and ERα in uterine leiomyoma and para-normal paraffin sections was detected by in situ hybridization and immunohistochemistry, and the relationship between the expression of microRNA-18a and ERα was evaluated. Results The expression of microRNA-18a in uterine fibroid tissue was significantly lower than that in the normal para-tumorous tissue (P <0.05), while the expression of ERα was significantly higher than that in normal para-tumorous tissue (P <0.05). There was a negative correlation between the expression of microRNA-18a and ERα in uterine fibroid tissue and adjacent normal smooth muscle. Conclusion microRNA-18a may play a significant role in the development of uterine fibroids by affecting the expression of ERα in uterine leiomyoma; microRNA-18a may be a new target for the treatment of uterine fibroids.