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目的 探讨脑胶质瘤中CDK4 、MTS基因异常及pRb表达改变与脑胶质瘤发生发展的相关性。方法 应用PCR SSCP、分子杂交及免疫组化技术检测 6 8例不同病理分级脑胶质瘤。结果 p16基因表达阴性或pRb无表达或CDK4 扩增多为单独发生 ,其中III IV级肿瘤中pRb、p16蛋白失表达或p15基因缺失或CDK4 基因扩增的综合发生率为 89% (4 2 / 47)。结论 脑胶质瘤中细胞生长周期“关卡”蛋白p16、pRb、或CDK4 单因素的异常比交错并发的改变更为常见 ,p16、pRb失活或CDK4 扩增的任一改变 ,即可能破坏细胞增殖的正常调控 ,促发脑胶质瘤细胞的癌性增殖或恶性演变
Objective To investigate the relationship between abnormal expression of CDK4, MTS gene and the expression of pRb in glioma and the development of glioma. Methods PCR SSCP, molecular hybridization and immunohistochemistry were used to detect 68 different pathological grading gliomas. Results The negative expression of p16 gene or no expression of pRb or the expansion of CDK4 occurred alone. The combined incidence of loss of expression of pRb and p16 protein or deletion of p15 gene or amplification of CDK4 gene in group III and IV tumors was 89% (4 2 / 47). Conclusions The single-factor abnormalities of p16, pRb, or CDK4 in the cell cycle of glioma cells are more common than the changes in staggered concurrency. Any change of p16, pRb inactivation or CDK4 amplification may destroy the cells The normal regulation of proliferation, promote cancerous proliferation or malignant progression of glioma cells