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采用免疫组化和银染色技术对31例肾盂癌组织中p53蛋白，核仁组成区嗜银蛋白（AgNOR）和增殖细胞核抗原（PCNA）进行研究。发现肾盂癌p53表达阳性率，AgNOR颗粒计数和PCNA增殖指数均随肿瘤病理分级、临床分期的上升而增加，且与预后呈负相关。p53蛋白阳性表达组AgNOR颗粒计数和PCNA增殖指数显著高于p53蛋白阴性表达组。肾盂癌AgNOR计数与PCNA增殖指数间存在正相关性。提示p53蛋白表达，AgNOR计数和PCNA免疫染色在肾孟癌生物学行为及预后评估中具有重要意义。
Immunohistochemistry and silver staining were used to study the expression of p53 protein, nucleolar organizer region AgNOR and proliferating cell nuclear antigen (PCNA) in 31 cases of renal pelvic carcinoma. It was found that the positive rate of p53 expression in renal pelvic cancer, AgNOR particle count and PCNA proliferation index with the tumor pathological grade, clinical stage increased and negatively correlated with the prognosis. The expression of AgNOR particles and PCNA in p53 positive group was significantly higher than that in p53 negative group. There was a positive correlation between AgNOR count and PCNA proliferation index in renal pelvic cancer. It is suggested that p53 protein expression, AgNOR count and PCNA immunostaining are important in the biological behavior and prognosis evaluation of renal cell carcinoma.