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应用聚合酶链反应（ＰＣＲ）检测６０例乙型肝炎患者外周血单个核细胞（ＰＢＭＣ）及血清ＨＢＶ·ＤＮＡ，阳性率分别为７６．７％与４５％。尤其在急性乙肝（ＡＨ）或抗ＨＢｅ阳生或抗ＨＢｓ阳性的患者中，ＨＢＶ·ＤＮＡ在ＰＢＭＣ中检出率显著高于血清。提示ＨＢＶ·ＤＮＡ普遍存在于乙肝患者甚ＩＨＢｓＡｇ转阴的病人ＰＢＭＣ中，可能是肝脏病变反复活动及病毒重复感染的来源．因而也导致既往有ＨＢＶ感染史的ＨＢｓＡｇ阴性献血员传播ＨＢＶ的可能。
The positive rate of HBV DNA in peripheral blood mononuclear cells (PBMCs) and serum HBV DNA in 60 patients with hepatitis B was detected by polymerase chain reaction (PCR). The positive rates were 76.7% and 45% respectively. Especially in acute hepatitis B (AH) or anti-HBe positive or anti-HBs positive patients, HBV DNA in PBMC detection rate was significantly higher than serum. Tip HBV · DNA ubiquitous in HBV patients with IHBsAg negative patients with PBMC may be repeated liver disease and repeated viral infection source. This also led to the possibility of HBV being transmitted by previous HBsAg-negative blood donors with a history of HBV infection.