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山西省虹鳟试验场用从日本引进的鱼卵孵化的虹鳟稚鱼暴发流行病,死亡率高达90％以上。经组织培养分离到病毒,能在鲑鳟细胞系中产生细胞病变,形成直径0.5—1mm的空斑。感染健康虹鳟稚鱼能复制出与天然发病相同的症状和死亡率。病毒对氯仿不敏感,耐酸、耐热。病毒负染后电镜观察为直径55—65mm的二十面体颗粒,无囊膜,具单层衣壳。经血清学鉴定为传染性胰脏坏死病病毒(Infectious pancreatic necrosis virus简称IPNV)在血清学交叉中和反应中与抗IPN-Sp株的抗血清有强烈的交叉反应,提示可能为IPN-Sp株。
Shanxi rainbow trout test site with rainbow trout hatching fish imported from Japan, juvenile outbreak of epidemics, the mortality rate as high as 90%. Tissue culture to isolate the virus, can produce cytopathic in salmon cell lines, the formation of 0.5-1 mm diameter plaque. Healthy rainbow trout juveniles replicate the same symptoms and mortality as their natural counterparts. The virus is not sensitive to chloroform, acid, heat. Negative staining after electron microscopy electron microscopy for the diameter of 55-65mm icosahedral particles, non-enveloped, with a single shell. Serological identification of Infectious pancreatic necrosis virus (IPNV) had a strong cross-reactivity with anti-IPN-Sp antiserum in the serological cross-neutralization reaction, suggesting that IPN-Sp strain .