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梨(Pyrus commumis L.,Barfleft—巴梨)植株可通过试管培养茎尖分生组织进行繁殖。嫩梢增殖的适宜BA(苄基腺嘌呤)浓度为5微克分子,将梢尖去除、放平,或倒转而减弱顶端优势时,也会促进嫩梢增殖。萘乙酸(NAA0.05微克分子)和赤霉酸(GA_31微克分子)对嫩梢增殖既无促进作用,也无抑制作用,在10微克分子NAA中培养3周后,生根的嫩梢转移至温室并定植于土壤中。
Pear (Pyrus commumis L., Barfleft-Barr) plants can be propagated by in vitro culture of shoot apical meristems. Appropriate proliferation of shoots BA (benzyl adenine) concentration of 5 micrograms, the tip will be removed, flattened, or inverted to weaken the top edge, it will also promote the proliferation of young shoots. Naphthaleneacetic acid (NAA 0.05 micromol) and gibberellic acid (GA 31 micromol) neither promoted nor inhibited the proliferation of shoots. After being cultured in 10 micromolar NAA for 3 weeks, the shoots were transplanted to the greenhouse And planted in the soil.