论文部分内容阅读
采用基因工程技术,在表达质粒 PTLIL-2中于人白细胞介素 2(interleukin 2,IL-2)cD-NA 片段前插入了来自质粒PDR540的含Tac 启动子的片段,并适当调整SD 序列与ATG 密码之间的距离,构建成含双Tac 启动子和双SD 序列的质粒 PTLIL-2DT。结果该重组质粒的IL-2 基因在大肠杆菌中表达效率提高了4倍。本文对重组 IL-2抽提和生物活性恢复的处理方法也作了探讨。
The Tac promoter-containing fragment from plasmid PDR540 was inserted in front of the human interleukin 2 (IL-2) cD-NA fragment in the expression plasmid PTLIL-2 by genetic engineering and the SD sequence was appropriately adjusted The distance between ATG codons was constructed as plasmid PTLIL-2DT with double Tac promoter and double SD sequence. Results The recombinant plasmid IL-2 gene expression efficiency in E. coli increased by 4 times. This article also explores the treatment of recombinant IL-2 extraction and recovery of biological activity.