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EB病毒引起鼻咽细胞癌变(始动因子)抑或对巴癌变的细胞起促进作用(促进因子),至今尚未有定论。不过癌前期增生细胞甚至正常鼻咽上皮细胞检出可能与细胞转化有关的EB病毒基因产物(EBNA)和EB病毒核酸的存在,是EB病毒在鼻咽癌变中起重要作用的线索。对于这种外加基因的进入方式,我们提出过不需通过病毒颗粒直接感染或细胞融合传递,可以通过DNA插入的基因转移方式进入宿主细胞。本文着重报导采用超声超滤方法和核酸原位杂交技术,分析EB病毒DNA的大分子和
Epstein-Barr virus (EBV) causes nasopharyngeal cell carcinogenesis (initiation factor) or promotes cancerous cells (promoting factors), but it has not yet been concluded. However, the presence of EBV1 and Epstein-Barr virus nucleic acids, which may be related to cell transformation, in precancerous proliferative cells or even normal nasopharyngeal epithelial cells is a clue that EBV plays an important role in nasopharyngeal carcinogenesis. With regard to the way in which this extra gene is introduced, we have proposed that it is not necessary to directly infect the virus particles or transfer the cell fusion, and it is possible to enter the host cell by means of gene transfer by DNA insertion. This article focuses on the use of ultrasound ultrafiltration and nucleic acid in situ hybridization to analyze the macromolecules of EBV DNA.