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Objective: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. Methods: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4 ℃ for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione S-transferase(GST), ascorbate oxidase, peroxidase, polyphenol oxidase(PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione(TRG) and lipid peroxidation(LPO). Results: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene(CDNB)-reduced glutathione(GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H_2O_2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. Conclusion: Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.
Objective: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. Methods: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000 × g at 4 ° C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase Results: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92 ± 6.80 μg / mg protein, GST 372.11 ± 5.70 μmol of 1 (non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation glutathione oxidized / min / mg protein, peroxidases 173.12 ± 9.40 μmol / g tissue, TRG 75.27 ± 3.55 μg / mg protein, SOD 58.03 ± 2.11 U / mg protein, CAT 46.70 ± 2.35 μmol of H 2 O consumed / min / mg protein in amount of ascorbate oxidase 17.41 ± 2.46 U / g tissue, LPO 2.71 ± 0.14 nmol / L of malondialdehyde formed / min / mg protein and PPO 1.14 ± 0.11 μmol / g tissue in moderate amount. Conclusion: Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causes diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.